|
| Status |
Public on Jul 08, 2011 |
| Title |
L3 glp-1 ash-2 #3 |
| Sample type |
RNA |
| |
|
| Source name |
whole worm L3 lysates
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
age: L3
genotype/variation: glp-1(e2141ts) mutant
treatment: ash-2 RNAi
|
| Treatment protocol |
Worms were washed several times with M9 buffer prior to extraction
|
| Growth protocol |
Worms were grown at 20 degrees
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAqueous kit (Ambion) was used
|
| Label |
biotin
|
| Label protocol |
Labeling was performed at the Stanford Protein and Nucleic Acid facility using biotin
|
| |
|
| Hybridization protocol |
Affymetrix protocol was followed by Stanford Protein and Nucleic Acid facility
|
| Scan protocol |
Again scanning was performed according to affymetrix protocol by Stanford Protein and Nucleic Acid facility
|
| Data processing |
Background adjustment and normalization was performed with RMA (Robust Multiarray Analysis) in R
|
| |
|
| Submission date |
Jul 08, 2011 |
| Last update date |
Jul 08, 2011 |
| Contact name |
Eric Lieberman Greer |
| E-mail(s) |
eric.greer@childrens.harvard.edu
|
| Organization name |
Stanford University
|
| Department |
Genetics
|
| Lab |
Anne Brunet
|
| Street address |
Alway M314 300 Pasteur Drive
|
| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE30505 |
Members of the H3K4 trimethylation complex regulate lifespan in a germline-dependent manner in C. elegans |
|
