|
| Status |
Public on Apr 28, 2024 |
| Title |
normal tissues of patient 4 |
| Sample type |
SRA |
| |
|
| Source name |
colon
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: colon
cell line: normal tissue
cell type: normal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Fresh normal mucosa and MCA tissue were washed with ice-cold PBS and minced into small pieces. Then the normal and tumor tissue pieces were digested with collagenase VIII at 0.38 mg/mL and DNase I at 0.1 mg/mL in 30% FBS for 1.0h at 37 °C. After digestion, tissue pieces were washed with PBS twice.
Library was constructed according to the manufacter's instructions (single cell 3' V2 protocol 10 X genomics)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
10 X genomics
|
| Data processing |
the demultiplexing, barcoded processing, gene counting and aggregation were conducted through the Cell Ranger Software V 3.1.0
Assembly: GRCH38
Supplementary files format and content: Tab-separated values files and matrix files
|
| |
|
| Submission date |
Jul 06, 2023 |
| Last update date |
Apr 28, 2024 |
| Contact name |
guangyong zheng |
| E-mail(s) |
zhenggy@sibs.ac.cn
|
| Organization name |
shutcm
|
| Street address |
cailun road 1200
|
| City |
Shanghai |
| ZIP/Postal code |
201203 |
| Country |
China |
| |
|
| Platform ID |
GPL18573 |
| Series (2) |
| GSE236696 |
Gene expression profile at single cell level of pair human mucinous colorectal adenocarcinoma(MCA) tissues and adjacent normal colon tissues |
| GSE236698 |
Mucinous colorectal adenocarcinoma |
|
| Relations |
| BioSample |
SAMN36339686 |
| SRA |
SRX20918235 |
