|
| Status |
Public on May 02, 2024 |
| Title |
mut_D10_rep3 |
| Sample type |
SRA |
| |
|
| Source name |
antenna
|
| Organism |
Harpegnathos saltator |
| Characteristics |
tissue: antenna
genotype: orco_mutant
sequencing batch: 2
time apf: D10
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Antennae were frozen with liquid nitrogen and ground into a fine dry powder using a pestle. RNA was extracted via a standard protocol using TRIzol Reagent, followed by ethanol precipitation. DNase treatment was performed in-solution, followed by a second round of TRIzol-chloroform and ethanol precipitation to remove the treatment.
We used the NEBNext Ultra II RNA Library Prep Kit for Illumina with an input of 110 ng RNA and 12 PCR cycles.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Mapping and counting was done using STAR v2.6.1d.
Count normalization was done using DESeq2 v1.34.0.
Assembly: Hsal_v8.6
Supplementary files format and content: tab-delimited text files that contain raw counts for each sample
Supplementary files format and content: comma-delimited text file that contains DESeq2-normalized counts for all samples
|
| |
|
| Submission date |
Jul 07, 2023 |
| Last update date |
May 02, 2024 |
| Contact name |
Bogdan Sieriebriennikov |
| E-mail(s) |
bs167@nyu.edu
|
| Organization name |
New York University
|
| Street address |
100 Washington Sq E
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10003 |
| Country |
USA |
| |
|
| Platform ID |
GPL22089 |
| Series (2) |
| GSE236792 |
Bulk gene expression profiles of pupal antennae in wild-type and orco mutant Harpegnathos saltator |
| GSE236793 |
Bulk gene expression profiles of adult and pupal antennae in wild-type and orco mutant Harpegnathos saltator |
|
| Relations |
| BioSample |
SAMN36363890 |
| SRA |
SRX20942362 |
