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Status |
Public on Aug 30, 2023 |
Title |
3_SBI_8 |
Sample type |
RNA |
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Source name |
Sprague Dawley, 3 days after simulated birth injury
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Organism |
Rattus norvegicus |
Characteristics |
strain: Sprague Dawley tissue: pubocaudalis rat skeletal muscle condition: 3 days after simulated birth injury
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Treatment protocol |
Simulated birth injury, saline or SKM injection as specified
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Extracted molecule |
total RNA |
Extraction protocol |
Pubocaudalis muscle was harvested, submerged in RNAlater and stored at -80C. Samples were then thawed and RNA was isolated with RNAeasy Fibrous Tissue Mini Kit.
|
Label |
Capture ProbeSet
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Label protocol |
RNA concentration was measured using a Qubit 3.0 Fluorometer with a Qubit™ RNA HS Assay kit. The hybridization buffer (70 µl) was then mixed with the Custom Reporter CodeSet solution, and 8 µl of this master mix was then added separately to 50-100 ng of RNA per tissue sample, and RNA-free water up to 13 µl total. Then, 2 µl of Capture ProbeSet was added to the mixture, thoroughly mixed and placed on a thermocycler at 65°C for 16-48 hours and then maintained at 4°C for less than 24 hours.
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|
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Hybridization protocol |
Using a two-step magnetic beads purification, probe excess was removed in PrepStation and target/probe complexes were bound on the cartridge.
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Scan protocol |
Nanostring nCounter Digital Analyzer processed digital barcode reads.
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Data processing |
Barcode reads were analyzed by nSolver Analysis Software 4.0 and differential expression analysis was done with a custom R script.
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Submission date |
Jul 11, 2023 |
Last update date |
Aug 30, 2023 |
Contact name |
Pamela Duran |
E-mail(s) |
paduran@ucsd.edu
|
Organization name |
University of California, San Diego
|
Street address |
2880 Torrey Pines Scenic Dr
|
City |
La Jolla |
ZIP/Postal code |
92037 |
Country |
USA |
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|
Platform ID |
GPL33563 |
Series (1) |
GSE237062 |
Pelvic floor muscle response after simulated birth injury and mechanisms of a skeletal muscle-derived ECM hydrogel (SKM) |
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