|
| Status |
Public on Jul 14, 2011 |
| Title |
[E-MTAB-513] HCT20148, skeletal muscle, male, 77 years, 2x50 paired end mRNA-seq |
| Sample type |
SRA |
| |
|
| Source name |
skeletal muscle, male, 77 years, 2x50 paired end mRNA-seq
|
| Organism |
Homo sapiens |
| Characteristics |
organismpart: skeletal muscle
Sex: male
age: 77 years
phenotype: caucasian
material type: total_RNA
libraryprep: mRNA-Seq
biosourceprovider: Human skeletal muscle total RNA, lot 046P021205086A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
nucleic_acid_extraction | 1ug of total RNA was subjected to two rounds of oligo-dT beads binding. Purified mRNA was fragmented and random primed for cDNA synthesis. cDNA fragements were end repared, added dATP, and liagted to illumina pair end sample prep adaptor. The ligated material was amplified by PCR for 15 cycles, and used for illumina sequencing.
sequencing | Illumina sequencing was performed at HiSeq 2000 at Illumina
bioassay_data_transformation | FASTQ files (Illumina format) of sequencing reads were retrieved from the sequencing machine
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
50bp_PE_mRNA_Seq_FCA_s_5_1_sequence
2x50 paired end mRNA-seq READ1
~ 0.5% phiX DNA spiked in
Performer: ILLUMINA-CA
|
| Data processing |
processed data not provided
|
| |
|
| Submission date |
Jul 12, 2011 |
| Last update date |
May 15, 2019 |
| Organization |
European Bioinformatics Institute |
| E-mail(s) |
miamexpress@ebi.ac.uk
|
| Lab |
ArrayExpress
|
| Street address |
Wellcome Trust Genome Campus
|
| City |
Hinxton |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB10 1SD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL11154 |
| Series (2) |
| GSE30611 |
[E-MTAB-513] Illumina Human Body Map 2.0 Project |
| GSE49417 |
The conserved organization of the human and mouse transcriptomes |
|
| Relations |
| Reanalyzed by |
GSE97211 |
| SRA |
ERX011212 |
