GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM759518 Query DataSets for GSM759518
Status Public on Jul 14, 2011
Title [E-MTAB-513] HCT20152, thyroid, female, 60 years, 1x75 single mRNA-Seq
Sample type SRA
Source name thyroid, female, 60 years, 1x75 single mRNA-Seq
Organism Homo sapiens
Characteristics organismpart: thyroid
Sex: female
age: 60 years
phenotype: caucasian
material type: total_RNA
libraryprep: mRNA-Seq
biosourceprovider: Human thyroid total RNA, lot 0908003
Extracted molecule total RNA
Extraction protocol nucleic_acid_extraction | 1ug of total RNA was subjected to two rounds of oligo-dT beads binding. Purified mRNA was fragmented and random primed for cDNA synthesis. cDNA fragements were end repared, added dATP, and liagted to illumina pair end sample prep adaptor. The ligated material was amplified by PCR for 15 cycles, and used for illumina sequencing.
sequencing | Illumina sequencing was performed at HiSeq 2000 at Illumina
bioassay_data_transformation | FASTQ files (Illumina format) of sequencing reads were retrieved from the sequencing machine
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Description 75bp_mRNA_Seq_FCB_s_8_sequence
1x75 single mRNA-Seq
~ 0.5% phiX DNA spiked in
Performer: ILLUMINA-CA
Data processing processed data not provided
Submission date Jul 12, 2011
Last update date May 15, 2019
Organization European Bioinformatics Institute
Lab ArrayExpress
Street address Wellcome Trust Genome Campus
City Hinxton
State/province Cambridgeshire
ZIP/Postal code CB10 1SD
Country United Kingdom
Platform ID GPL11154
Series (1)
GSE30611 [E-MTAB-513] Illumina Human Body Map 2.0 Project
Reanalyzed by GSE97211
SRA ERX011213

Supplementary data files not provided
SRA Run SelectorHelp
Processed data not provided for this record
Raw data are available in SRA

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap