|
| Status |
Public on Jul 14, 2011 |
| Title |
[E-MTAB-513] HCT20173, 16 Tissues mixture, 100bp_Stranded_RNA_Seq_FCB_s_5_sequence |
| Sample type |
SRA |
| |
|
| Source name |
16 Tissues mixture, 100bp_Stranded_RNA_Seq_FCB_s_5_sequence
|
| Organism |
Homo sapiens |
| Characteristics |
organismpart: 16 Tissues mixture
material type: molecular_mixture
libraryprep: totalRNA-stranded+DSN
|
| Extracted molecule |
total RNA |
| Extraction protocol |
nucleic_acid_extraction | 100ng of total RNA was fragmentated, end repared and ligated with illumina small RNA v1.5 adaptors. The ligated RNA was reverse transcribed and PCR amplified with 15 cycles. The PCR product was normalized by DSN digestion and used for sequencing.
sequencing | Illumina sequencing was performed at HiSeq 2000 at Illumina
bioassay_data_transformation | FASTQ files (Illumina format) of sequencing reads were retrieved from the sequencing machine
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
100bp_Stranded_RNA_Seq_FCB_s_5_sequence
1x100 stranded total transcriptome preps; the insert size is variable; could be less than 100 bp. The linker sequence at the end of read will be ATCTCGTATGCCGTCTTCTGCTTG.
~ 0.5% phiX DNA spiked in
Performer: ILLUMINA-CA
|
| Data processing |
processed data not provided
|
| |
|
| Submission date |
Jul 12, 2011 |
| Last update date |
May 15, 2019 |
| Organization |
European Bioinformatics Institute |
| E-mail(s) |
miamexpress@ebi.ac.uk
|
| Lab |
ArrayExpress
|
| Street address |
Wellcome Trust Genome Campus
|
| City |
Hinxton |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB10 1SD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE30611 |
[E-MTAB-513] Illumina Human Body Map 2.0 Project |
|
| Relations |
| SRA |
ERX011185 |
