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| Status |
Public on Jul 15, 2023 |
| Title |
DNA tur1delta 30E rep 3 |
| Sample type |
SRA |
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| Source name |
NE1602
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| Organism |
Cryptococcus neoformans |
| Characteristics |
cell line: NE1602
genotype: tur1delta
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| Growth protocol |
Cells were grown on YPD at 30°C in exponential phase. The Cryptococcus cell preparation was spiked in with one tenth (OD/OD) of S. cerevisiae strain FY834 cells grown in YPD at 30°C in expontial phase.
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
none provided
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| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
NextSeq 550 |
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| Data processing |
150 bases were sequenced from both ends using an Illumina NextSeq500 instrument according to the manufacturer’s instructions (Illumina).
The reads were then cleaned using cutadapt/1.18. Trimmed reads were mapped to the C. neoformans H99 and to the S. cerevisiae S288C genomes with Bowtie2 using default settings. For each sample, total DNA and RNA reads counts aligned to each organism were used to calculate an alpha normalization factor.
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| Submission date |
Jul 13, 2023 |
| Last update date |
Jul 15, 2023 |
| Contact name |
Guilhem Janbon |
| E-mail(s) |
janbon@pasteur.fr
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| Organization name |
Institut Pasteur
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| Street address |
25 rue du Dr Roux
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| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
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| Platform ID |
GPL33584 |
| Series (1) |
| GSE237320 |
Tur1 regulates alternative TSS usage in Cryptococcus |
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| Relations |
| BioSample |
SAMN36439099 |
| SRA |
SRX21012748 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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