|
Status |
Public on Oct 14, 2011 |
Title |
normal wild-type E18.5 lungs, technical replicate 2 |
Sample type |
RNA |
|
|
Source name |
E18.5 lung
|
Organism |
Mus musculus |
Characteristics |
strain: c57Bl6/J age: E18.5 embryos tissue: lung diet: control chow
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
technical replicate 2
|
Data processing |
The data were normalized using cubic spline in Illumina GenomeStudio and also using the VAMPIRE Bayesian model with a 2-sided Bonferroni-corrected threshold of 0.05.
|
|
|
Submission date |
Jul 14, 2011 |
Last update date |
Oct 14, 2011 |
Contact name |
Ruth T Yu |
Organization name |
Salk Institute
|
Department |
Gene Expression Lab
|
Lab |
Ronald Evans
|
Street address |
10010 N Torrey Pines Rd
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92037 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE30661 |
SMRT-RID mutation linked to Type I Pneumocyte Associated Respiratory Distress Syndrome via TR repression |
|