|
Status |
Public on Dec 24, 2011 |
Title |
HCC1937, replicate2 |
Sample type |
RNA |
|
|
Source name |
HCC1937
|
Organism |
Homo sapiens |
Characteristics |
cell line: HCC1937 cell type: breast cancer cells brca1 expression: no
|
Growth protocol |
Cell lines were grown in 10% FBS DMEM media with 2% penicillin/streptomycin at 37ºC, 5% CO2 atmosphere and 80% humidity.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from cell pellets using Trizol following the manufacturer's recommendations.
|
Label |
Hy3
|
Label protocol |
300ng of total RNA were treated with Calf Intestine Phosphatase and than labeled with Hy3 fluorescent dye.
|
|
|
Hybridization protocol |
Exiqon miRNA microarray slides containing denaturated labeled RNA in 1x hybridization buffer were hybridized at 56ºC for 16h.
|
Scan protocol |
Slides were scanned using an Agilent array scanner set to 10μm wit 100PMT sensitivity in an ozone-free-environment. Fluorescence intensities on scanned images were quantified using Feature Extraction software (Agilent) using the modified Exiqon protocol.
|
Description |
No BRCA1 expression confirmed by Western Blot.
|
Data processing |
Background correction was carried out using normexp, and data was normalized using quantiles between-arrays normalization. Data processing was carried out using Bioconductor´s limma package.
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|
|
Submission date |
Jul 18, 2011 |
Last update date |
Dec 24, 2011 |
Contact name |
Beatriz Martinez |
E-mail(s) |
bmartinez@cnio.es
|
Organization name |
Spanish National Cancer Research Centre (CNIO)
|
Department |
Human Cancer Genetics
|
Street address |
Calle Melchor Fernandez Almagro 3
|
City |
Madrid |
ZIP/Postal code |
28029 |
Country |
Spain |
|
|
Platform ID |
GPL7723 |
Series (2) |
GSE30763 |
Integration of BRCA1-mediated miRNA and mRNA signatures reveal miR-146a, miR-99b and miR-205 regulation of the TRAF2 and NFkB pathways (miRNA dataset) |
GSE30822 |
Integration of BRCA1-mediated miRNA and mRNA signatures reveal miR-146a, miR-99b and miR-205 regulation of the TRAF2 and NFkB pathways |
|