|
| Status |
Public on Jul 23, 2023 |
| Title |
traditional breeding [TB-3] |
| Sample type |
SRA |
| |
|
| Source name |
duodenum
|
| Organism |
Anas platyrhynchos |
| Characteristics |
tissue: duodenum
cell type: Intestinal epithelial cells
group: traditional breeding
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of each sample was extracted with Trizol (Invitrogen, USA). Using a Novex 15% TBE-Urea gel, 20–30-nt long RNA fragments were isolated and purified from 10 μg of total RNA
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
apla intestine
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
After removing the adaptors, low quality tags and contaminants reads, the clean reads were aligned with the duck genome
The expressions of miRNAs were normalized by Fragments per Kilobase of Transcript per Million Fragments Mapped (FPKM)
Supplementary files format and content: FPKM
|
| |
|
| Submission date |
Jul 18, 2023 |
| Last update date |
Jul 23, 2023 |
| Contact name |
Li Zhang |
| E-mail(s) |
ZL15182464705tr@gmail.com
|
| Organization name |
Institute of Animal Husbandry and Veterinary Medicine, Hubei Academy of Agricultural Sciences
|
| Street address |
No. 29, Nanhu Avenue, Shizishan Street, Hongshan District, Wuhan City, Hubei Pro
|
| City |
Wu han |
| State/province |
Hu bei |
| ZIP/Postal code |
430064 |
| Country |
China |
| |
|
| Platform ID |
GPL19739 |
| Series (1) |
| GSE237634 |
miRNA profile of duck duodenum mucosa damage caused by oxidative stress |
|
| Relations |
| BioSample |
SAMN36534291 |
| SRA |
SRX21075833 |
