|
Status |
Public on Oct 31, 2023 |
Title |
desmoid tumor, DES934 |
Sample type |
RNA |
|
|
Source name |
desmoid tumor
|
Organism |
Homo sapiens |
Characteristics |
tissue: desmoid tumor treatment: none tumor grade: low
|
Treatment protocol |
none
|
Growth protocol |
not applicable (tissue samples)
|
Extracted molecule |
total RNA |
Extraction protocol |
Cryomolds were prepared from tissue samples and macrodissected after evaluation by a sarcoma specific pathologist. After histologic confirmation, RNA was prepared using Rneasy kits from QIAGEN and hybridization to U133A_2.0 gene expression arrays performed per standard protocols
|
Label |
biotin
|
Label protocol |
cDNA was prepared by using oligo(dT)24-T7 (Genset Corp.), and cRNA was prepared by using biotinylated UTP and CTP
|
|
|
Hybridization protocol |
cRNA was hybridized to U133A_2.0 expression arrays
|
Scan protocol |
Fluorescent signal was measured by laser confocal scanning (Agilent) and converted to signal intesity (Affymetrix Microarray Suite)
|
Data processing |
CEL files were processed and normalized using gcrma methods implemented in the BioConductor package gcrma. Citation: Wu J, Gentry RIwcfJMJ (2023). gcrma: Background Adjustment Using Sequence Information. R package version 2.72.0.
|
|
|
Submission date |
Jul 18, 2023 |
Last update date |
Oct 31, 2023 |
Contact name |
Bic MSKCC |
Organization name |
Memorial SLoan-Kettering Cancer Center
|
Street address |
1275 York Ave.
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10021 |
Country |
USA |
|
|
Platform ID |
GPL571 |
Series (2) |
GSE237692 |
PDGFRβ signaling cooperates with β-catenin to modulate c-Abl and biologic behavior of desmoid-type fibromatosis [microarray] |
GSE239409 |
PDGFRβ signaling cooperates with β-catenin to modulate c-Abl and biologic behavior of desmoid-type fibromatosis |
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