|
| Status |
Public on Oct 31, 2023 |
| Title |
normal fat, NF8293 |
| Sample type |
RNA |
| |
|
| Source name |
normal fat
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: normal fat
treatment: none
tumor grade: not applicable
|
| Treatment protocol |
none
|
| Growth protocol |
not applicable (tissue samples)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cryomolds were prepared from tissue samples and macrodissected after evaluation by a sarcoma specific pathologist. After histologic confirmation, RNA was prepared using Rneasy kits from QIAGEN and hybridization to U133A_2.0 gene expression arrays performed per standard protocols
|
| Label |
biotin
|
| Label protocol |
cDNA was prepared by using oligo(dT)24-T7 (Genset Corp.), and cRNA was prepared by using biotinylated UTP and CTP
|
| |
|
| Hybridization protocol |
cRNA was hybridized to U133A_2.0 expression arrays
|
| Scan protocol |
Fluorescent signal was measured by laser confocal scanning (Agilent) and converted to signal intesity (Affymetrix Microarray Suite)
|
| Data processing |
CEL files were processed and normalized using gcrma methods implemented in the BioConductor package gcrma. Citation: Wu J, Gentry RIwcfJMJ (2023). gcrma: Background Adjustment Using Sequence Information. R package version 2.72.0.
|
| |
|
| Submission date |
Jul 18, 2023 |
| Last update date |
Oct 31, 2023 |
| Contact name |
Bic MSKCC |
| Organization name |
Memorial SLoan-Kettering Cancer Center
|
| Street address |
1275 York Ave.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (2) |
| GSE237692 |
PDGFRβ signaling cooperates with β-catenin to modulate c-Abl and biologic behavior of desmoid-type fibromatosis [microarray] |
| GSE239409 |
PDGFRβ signaling cooperates with β-catenin to modulate c-Abl and biologic behavior of desmoid-type fibromatosis |
|
