|
| Status |
Public on May 22, 2024 |
| Title |
sample4_day2_differentiation_medium |
| Sample type |
SRA |
| |
|
| Source name |
Monocytes/macrophages
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Monocytes/macrophages
genotype: Retrovirally transduced
treatment: Differentiation
|
| Growth protocol |
Cultivation on cell culture 24 well plates in IMDM containing 20% FCS, 1% Penicillin/Streptomycin, 2 mM L-Glutamine with:
complete medium: 10 ng/ml IL-3, 20 ng/ml IL-6, 20 ng/ml SCF, 20 ng/ml TPO, 20 ng/ml FLT3-L, 20 ng/ml GM-CSF
differentiation medium: 20 ng/ml GM-CSF, 20 ng/ml IFNγ, 100 ng/ml LPS
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Cells were harvested via centrifugation and dilution to 1x106 cells/ml in PBS with 1% BSA. All samples had a viability of >95%. Cells were prepared according to the Handbook "Cell Preparation for Single Cell Protocols" by 10x Genomics.
10x Genomics Single Cell 3' v3
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
NextSeq 2000 |
| |
|
| Description |
Sample_4_TTGAGAGT:CTACCAGG
|
| Data processing |
demultiplexed data were aligned and quantified using cellranger v7.1.0 using GRCh38 reference genome v2020
Assembly: GRCh38
Supplementary files format and content: HDF5 Feature-Barcode Matrix Format. Binary format of multidimensional arrays containing count of reads containing valid barcodes and UMIs as well as metadata on the libraries and gene sets/annotations.
|
| |
|
| Submission date |
Jul 20, 2023 |
| Last update date |
May 22, 2024 |
| Contact name |
Anna Danese |
| Organization name |
Ludwig Maximilians University
|
| Street address |
Grosshaderner strasse 9
|
| City |
Planegg |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
|
| Platform ID |
GPL30173 |
| Series (1) |
| GSE237826 |
Engineering an inducible leukemia-associated transcription factor enables large-scale ex vivo production of functional human phagocytes |
|
| Relations |
| BioSample |
SAMN41301119 |
| SRA |
SRX24512760 |
