|
Status |
Public on May 23, 2024 |
Title |
Astrocyte day21 rep1 |
Sample type |
SRA |
|
|
Source name |
cerebral cortex
|
Organism |
Mus musculus |
Characteristics |
tissue: cerebral cortex cell type: Astrocyte genotype: WT C57BL/6J treatment: ischemic stroke
|
Treatment protocol |
The distal middle cerebral artery (MCA) was permanently ligated with 10-0 sutures followed by 7 min of occlusion of the bilateral common carotid artery (CCA). The sham control group only received skull surface lesions.
|
Extracted molecule |
total RNA |
Extraction protocol |
Astrocytes were isolated via FACS, and RNA was harvested using Qiagen RNeasy MinElute Cleanup Kit (Cat NO. 74104) RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 4000 |
|
|
Data processing |
Illumina Casava1.8 software used for basecalling. Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using bowtie v2.3.4.2 The read numbers of the corresponding genes were counted via HTSeq v0.6.1. Assembly: mm10 Supplementary files format and content: tab-delimited text files includes raw conuts for each Sample
|
|
|
Submission date |
Jul 24, 2023 |
Last update date |
May 23, 2024 |
Contact name |
Song Wang |
Organization name |
Beijing Friendship Hospital, Capital Medical University
|
Street address |
NO.95, Yong'An Road, Xicheng District
|
City |
Beijing |
ZIP/Postal code |
100050 |
Country |
China |
|
|
Platform ID |
GPL21103 |
Series (1) |
GSE238125 |
Dynamic astrocytic gene expression profiles after ischemic stroke |
|
Relations |
BioSample |
SAMN36695341 |
SRA |
SRX21139328 |