|
| Status |
Public on Mar 01, 2024 |
| Title |
WT_CDAHFD_2 |
| Sample type |
genomic |
| |
|
| Source name |
liver
|
| Organism |
Mus musculus |
| Characteristics |
Sex: male
strain: C57BL/6J
tissue: liver
treatment: CDAHFD
genotype: WT
|
| Treatment protocol |
7-week old male hepatocyte-specific PPARα KO and WT mice were fed either a chow diet (normal standard diet, containing 9% energy from fat, 58% from carbohydrates, and 33% from protein) or a CDAHFD (choline-deficient L-amino acid defined high-fat diet, A06071302, New Brunswick, NJ USA, containing 62% energy from fat, 20% from carbohydrates, and 18% from protein) for 6 weeks ad libitum
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
At 13 weeks, the mice were sacrificed by cervical dislocation after anaesthesia with ketamine and xylazine diluted in phosphate-buffered saline (PBS) (2:2:6). Liver samples were immediately snap frozen and genomic DNA was extracted and purified from liver biopsies using Qiagen DNeasy Kit according to standard instructions
|
| Label |
Cy3 and Cy5
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Mouse Methylation BeadChip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using Illumina iScan using standard recommended Illumina scanner setting
|
| Description |
205785610003_R01C02
|
| Data processing |
R studio (v. 4.2.0) via the Enmix package
|
| |
|
| Submission date |
Jul 24, 2023 |
| Last update date |
Mar 01, 2024 |
| Contact name |
Claudia Theys |
| E-mail(s) |
claudia.theys@uantwerpen.be
|
| Organization name |
Universiteit Antwerpen
|
| Department |
FBD
|
| Lab |
PPES
|
| Street address |
Universiteitsplein,1
|
| City |
Wilrijk |
| State/province |
Antwerpen (nl) |
| ZIP/Postal code |
2610 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL32685 |
| Series (2) |
| GSE238173 |
Loss of PPARα function promotes epigenetic dysregulation of lipid homeostasis driving ferroptosis and pyroptosis lipotoxicity in Metabolic Dysfunction Associated Steatotic Liver Disease (MASLD) [MouseMethylation] |
| GSE239279 |
Loss of PPARα function promotes epigenetic dysregulation of lipid homeostasis driving ferroptosis and pyroptosis lipotoxicity in Metabolic Dysfunction Associated Steatotic Liver Disease (MASLD) |
|
