|
| Status |
Public on Feb 27, 2024 |
| Title |
E12.5M_1_1_EZdirect_Low_WorkflowJ_19 |
| Sample type |
genomic |
| |
|
| Source name |
E12.5, male, Primordial germ cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Primordial germ cells
input_ng: Low number of PGCs
|
| Growth protocol |
After obtaining cells from ATCC, they were cultured according to the instructions provided in the product sheet
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated using The DNeasy Blood and Tissue Kit (Qiagen, 69504) with the manufactural protocols
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Standard Illumina Protocol
|
| Scan protocol |
Standard Illumina Protocol
|
| Description |
Testing MM285 Array on low input DNA with PGCs
207097260023_R03C02
|
| Data processing |
SeSAMe software v1.18
Normalized average Beta
Unmethylated and methylated signal intensities
|
| |
|
| Submission date |
Jul 26, 2023 |
| Last update date |
Feb 27, 2024 |
| Contact name |
Wanding Zhou |
| E-mail(s) |
ZHOUW3@chop.edu
|
| Organization name |
Children's Hospital of Philadelphia
|
| Department |
Pathology & Lab Medicine
|
| Lab |
Zhou Lab
|
| Street address |
3501 Civic Center Blvd, Philadelphia, PA 19104, Colket Translational Research Building, 9300, Colket Translational Research Building
|
| City |
Phialdelphia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL30650 |
| Series (1) |
| GSE239290 |
Optimizing the experimental and computational methods for Infinium DNA methylation BeadChip on low input and single cells |
|
