|
| Status |
Public on Nov 01, 2024 |
| Title |
GM1_crossfostered_sample25 |
| Sample type |
genomic |
| |
|
| Source name |
Hippocampus
|
| Organism |
Mus musculus |
| Characteristics |
experimental group: GM1CF
gut microbiota: GM1
class: crossfostered
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated from the hippocampal tissue using the DNeasy Kit (Qiagen, Valencia, CA) following the manufacturer’s instructions.
|
| Label |
Cy3, Cy5
|
| Label protocol |
Standard Illumina labelling protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Mouse Methylation BeadChip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using High Scan SQ using standard recommended Illumina scanner setting
|
| Description |
Genomic DNA isolated from hippocampus of murine mice
|
| Data processing |
Microarray data was processed using the ENmix version.1.34.02 (Xu et al, 2016) and minfi v.1.44.0 (Aryee et al, 2014packages
Average Beta or normalized (provide the normalization method) Average Beta
Unmethylated and methylated signal intensities
|
| |
|
| Submission date |
Jul 26, 2023 |
| Last update date |
Nov 01, 2024 |
| Contact name |
Rene Gabriel Cortese |
| E-mail(s) |
rene.cortese@health.missouri.edu
|
| Phone |
5738844081
|
| Organization name |
University of Missouri
|
| Department |
Child health Research Institute
|
| Street address |
1 Hospital Dr. MA104E
|
| City |
Columbia |
| State/province |
MO |
| ZIP/Postal code |
65211 |
| Country |
USA |
| |
|
| Platform ID |
GPL32685 |
| Series (1) |
| GSE239371 |
Fetal programming by the maternal microbiome of offspring behavior, and DNA methylation and gene expression within the hippocampus |
|
