|
| Status |
Public on Apr 18, 2024 |
| Title |
Levels of miRNAs in the plasma exosomes of mice bearing A549Ago2-KO/HA-Ago4Δ (Dm) tumors |
| Sample type |
RNA |
| |
|
| Source name |
miRNA from DM mice plasma exosome
|
| Organism |
Mus |
| Characteristics |
cell type: plasma exosome
genotype: A549Ago2-KO/HA-Ago2Δ (Dm) tumors
|
| Treatment protocol |
A549Ago2-KO/Ago2Wt and A549Ago2-KO/Ago2Δ cells were subcutaneously implanted in SCID mice. All mice that were injected with either 1 × 106 A549Ago2-KO/Ago2Wt. Plasma exosomes were collected and total RNA (included miRNA) werw extracted by Trizol reagent
|
| Growth protocol |
Animals complied with protocols approved by the NHRI Committee on Animal Care
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated miRNA were prepared using FlashTag Biotin HSR RNA Labeling Kit.
|
| |
|
| Hybridization protocol |
21.5 μLof Biotin-labeled sample were hybridized for 16 hr at 48℃ on miRNA 3.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 7G.
|
| Description |
miRNA expression data from mice plasma exosomes
|
| Data processing |
Affymetrix GeneChip Command Console software (version4.0, Affymetrix) was used to analyze array images to get raw data and then Affymetrix Expression Console 1.3.1. for normalization; Partek Genomics Suite software for statistical analysis
|
| |
|
| Submission date |
Jul 28, 2023 |
| Last update date |
Apr 18, 2024 |
| Contact name |
LU LI YU |
| E-mail(s) |
n27293031@gmail.com
|
| Organization name |
ational Health Research Institutes
|
| Street address |
35, Keyan Road, Zhunan Town,
|
| City |
Miaoli County |
| ZIP/Postal code |
350 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE239599 |
plasma exosome from mice bearing Ago2wt and Ago2dm tumor |
|
