|
| Status |
Public on Nov 26, 2023 |
| Title |
Wt-Ovary5_350_227_S3 |
| Sample type |
SRA |
| |
|
| Source name |
ovary
|
| Organism |
Anopheles gambiae |
| Characteristics |
tissue: ovary
genotype: mosGILT WT
treatment: Blood feeding
|
| Treatment protocol |
mosGILTnull and Wild type (WT,control) mosquitoes were fed on naïve mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The whole body, ovaries, and fat bodies were collected from blood-fed mosquitoes 24 h after completion of the meal. Total RNA was extracted from the whole body, ovaries, or fat bodies from mosquitoes. Trizol was added to the tissues and RNA was isolated using a RNeasy Mini Kit (QIAGEN)
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Partek Flow
RNAseq data were trimmed and aligned to the Anopheles genome, with associated annotation file using STAR (v2.7.3a).
The aligned reads were quantified to Ensembl transcripts using the Partek E/M algorithm and the subsequent steps were performed on gene-level annotation followed by total count normalization.
The counts generated were processed by gene set analysis (GSA).
Assembly: Anopheles gambiae PEST
Supplementary files format and content: tab-delimited text files include baseMean for each gene
|
| |
|
| Submission date |
Jul 28, 2023 |
| Last update date |
Nov 26, 2023 |
| Contact name |
Xiaotian Tang |
| E-mail(s) |
tangxt@zju.edu.cn
|
| Organization name |
Zhejiang University
|
| Department |
Entomology
|
| Street address |
Agro-Bio-Environ Building, Zhejiang University
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310030 |
| Country |
China |
| |
|
| Platform ID |
GPL32063 |
| Series (1) |
| GSE239600 |
Anopheles gambiae mosGILT regulates innate immune genes and zpg expression |
|
| Relations |
| BioSample |
SAMN36749469 |
| SRA |
SRX21185673 |
