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Sample GSM7669564 Query DataSets for GSM7669564
Status Public on Oct 04, 2023
Title PATIENT 27739PH
Sample type RNA
 
Source name Monocytes CD14+
Organism Homo sapiens
Characteristics tissue: Peripheral blood
cell type: Monocytes CD14+
condition: excessive alcohol drinker
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from CD14+ isolated monocytes and monocyte-derived macrophages using the miRNA easy Micro Kit (Qiagen), following the manufacturer’s instructions. Briefly, RNA was extracted using a phenol-chloroform mixture, precipitated in ethanol, and purified using RNase-free columns.
Label Hy3
Label protocol 1µg of total RNA was labelled with the Exiqon Kit protocol following the manufacturer´s instructions.
 
Hybridization protocol Samples were heated at 95º C in darkness, hybridized, and washed with the robotic HS 4800 Pro system (Tecan®).
Scan protocol Fluorescence was scanned with a GenePix 4000B (Axon Instruments™) microarray scanner. Raw data processing was performed with the
Data processing Image processing was performed using Gene Prix Pro (v. 6.0), and data processing was performed using MEV (MultiExperiment Viewer) and EXpander 6.0
 
Submission date Jul 31, 2023
Last update date Oct 04, 2023
Contact name Juan Luis Garcia
E-mail(s) jlgarcia@usal.es
Phone 923294812
Organization name CIC/IBSAL
Street address Campus Miguel de Unamuno Centro de Investigacion del Cancer, IBSAL
City Salamanca
State/province Type Below
ZIP/Postal code 37007
Country Spain
 
Platform ID GPL11434
Series (1)
GSE239659 Increased pro-inflammatory phenotype of peripheral blood monocytes and monocyte-derived macrophages among patients with excessive ethanol intake

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
46202 1.241387496
146014 7.99E-15
146185 -0.349779324
17415 7.11E-15
42485 -0.025634294
42545 0.029357514
17283 -0.160101621
17306 -0.004981368
27537 1.059693276
42882 -0.109855561
17519 -0.013195173
17623 0.001155989
17655 -0.238158013
42897 -0.032527817
17541 1.27E-13
17358 1.038013931
42773 -0.00010207
46818 -8.88E-15
42466 1.10522281
17657 0.245271506

Total number of rows: 2365

Table truncated, full table size 31 Kbytes.




Supplementary file Size Download File type/resource
GSM7669564_27739.gpr.gz 947.7 Kb (ftp)(http) GPR
Processed data included within Sample table

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