|
| Status |
Public on Oct 04, 2023 |
| Title |
PATIENT 27700PH |
| Sample type |
RNA |
| |
|
| Source name |
Monocytes CD14+
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Peripheral blood
cell type: Monocytes CD14+
condition: excessive alcohol drinker
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from CD14+ isolated monocytes and monocyte-derived macrophages using the miRNA easy Micro Kit (Qiagen), following the manufacturer’s instructions. Briefly, RNA was extracted using a phenol-chloroform mixture, precipitated in ethanol, and purified using RNase-free columns.
|
| Label |
Hy3
|
| Label protocol |
1µg of total RNA was labelled with the Exiqon Kit protocol following the manufacturer´s instructions.
|
| |
|
| Hybridization protocol |
Samples were heated at 95º C in darkness, hybridized, and washed with the robotic HS 4800 Pro system (Tecan®).
|
| Scan protocol |
Fluorescence was scanned with a GenePix 4000B (Axon Instruments™) microarray scanner. Raw data processing was performed with the
|
| Data processing |
Image processing was performed using Gene Prix Pro (v. 6.0), and data processing was performed using MEV (MultiExperiment Viewer) and EXpander 6.0
|
| |
|
| Submission date |
Jul 31, 2023 |
| Last update date |
Oct 04, 2023 |
| Contact name |
Juan Luis Garcia |
| E-mail(s) |
jlgarcia@usal.es
|
| Phone |
923294812
|
| Organization name |
CIC/IBSAL
|
| Street address |
Campus Miguel de Unamuno Centro de Investigacion del Cancer, IBSAL
|
| City |
Salamanca |
| State/province |
Type Below |
| ZIP/Postal code |
37007 |
| Country |
Spain |
| |
|
| Platform ID |
GPL11434 |
| Series (1) |
| GSE239659 |
Increased pro-inflammatory phenotype of peripheral blood monocytes and monocyte-derived macrophages among patients with excessive ethanol intake |
|
