|
| Status |
Public on Mar 28, 2024 |
| Title |
REL-KO human Tconv, donor 1 |
| Sample type |
SRA |
| |
|
| Source name |
Human blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Human blood
individual: donor 1
cell type: CD4+CD25- T cells
genotype: REL-/-
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was isolated with Nucleospin RNA extraction kits (Macherey Nagel)
libraries were prepared using an Illumina TruSeq Library Kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
REL_KO3_guide1
|
| Data processing |
Bcl files were converted to fastq using Bcl2fastq (2.20.0).
Gene expression was quantified using Salmon (0.14.1) and the annotation of protein coding genes from gencode v29.
Transcription matrix was done using R package tximport on the salmon results.
Assembly: hg38
Supplementary files format and content: tab delimited file with samples in columns, genes in rows, and estimated raw counts.
|
| |
|
| Submission date |
Jul 31, 2023 |
| Last update date |
Mar 28, 2024 |
| Contact name |
Yenkel Grinberg-Bleyer |
| E-mail(s) |
yenkel.grinberg-bleyer@lyon.unicancer.fr
|
| Organization name |
Cancer Research Center of Lyon
|
| Department |
Immunity, viruses and inflammation
|
| Lab |
INSERM U1052
|
| Street address |
28 rue Laennec
|
| City |
Lyon |
| ZIP/Postal code |
69008 |
| Country |
France |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE239698 |
NF-κB transcription factors RelA and c-Rel selectively control CD4+ T-cell function in multiple sclerosis and cancer [bulk_human] |
| GSE239704 |
NF-κB transcription factors RelA and c-Rel selectively control CD4+ T-cell function in multiple sclerosis and cancer |
|
| Relations |
| BioSample |
SAMN36776158 |
| SRA |
SRX21202542 |
