|
| Status |
Public on Oct 02, 2023 |
| Title |
Case 10 |
| Sample type |
RNA |
| |
|
| Source name |
human plasma
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: Metabolic syndrome
gender: Female
tissue: Plasma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from 1 mL plasma using Trizol reagent following the manufacturer’s instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
FlashTag Biotin HSR RNA labeling kit
|
| Scan protocol |
Microarrays were scanned using the Affymetrix GeneChip Command Console.
|
| Description |
Small RNA expression data in human plasma
PlasmaMiRNA MetSyndrome
|
| Data processing |
Data were analyzed in the R/Bioconductor environment with the software Oligo, pd.mirna.3.0 and Linear Models for Microarray Analysis (LIMMA).
|
| |
|
| Submission date |
Aug 02, 2023 |
| Last update date |
Oct 02, 2023 |
| Contact name |
M Elizabeth Tejero |
| E-mail(s) |
etejero@inmegen.gob.mx
|
| Phone |
+525513912583
|
| Organization name |
Instituto Nacional de Medicina Genomica, Mexico City
|
| Street address |
Periferico sur 4809
|
| City |
Tlalpan |
| State/province |
Ciudad de México |
| ZIP/Postal code |
14160 |
| Country |
Mexico |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE239866 |
MicroRNAs in plasma in metabolic syndrome. |
|
