|
Status |
Public on Aug 23, 2023 |
Title |
Negative Control group, NC_3 |
Sample type |
SRA |
|
|
Source name |
Primary cells
|
Organism |
Oryctolagus cuniculus |
Characteristics |
cell type: Parathyroid cell genotype: WT treatment: none time: Day0
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was harvested using RNA Quick Purification kit (ESscience). 1.1ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using TruePrep RNA Library Prep Kit for Illumina (Vazyme)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
CLC Genomics Workbench v11.0.1 Sequence reads were trimmed for adaptor sequence/low-quality sequence using CLC genomic benchw ork (parameter-Qualitu limit:0.05) Trimmed sequence reads were mapped to GrCM38/mm10 using CLC genomic benchw ork (parameters-mismath cost:2 insertion cost:3 deletion cost:3 length fraction:0.8 similarity fration:0.8) Read count extraction and normalization were performed using CLC genomic benchw ork Supplementary files format and content: tab-delimited text file include FPKM for each sample
|
|
|
Submission date |
Aug 04, 2023 |
Last update date |
Aug 23, 2023 |
Contact name |
Nianqiu Liu |
E-mail(s) |
20201650@kmmu.edu.cn
|
Organization name |
The Third Affiliated Hospital of Kunming Medical University: Yunnan Cancer Center, Kunming, 650000, Yunnan
|
Department |
Breast Surgery
|
Street address |
519 Kunzhou Road, Xishan
|
City |
Kunming |
State/province |
China/Yunnan |
ZIP/Postal code |
650118 |
Country |
China |
|
|
Platform ID |
GPL21255 |
Series (1) |
GSE240162 |
Gallic Acid Pretreatment Mitigates Parathyroid Ischemia-Reperfusion Injury through Signaling Pathway Modulation |
|
Relations |
BioSample |
SAMN36844480 |
SRA |
SRX21256698 |