 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Aug 23, 2023 |
| Title |
Model group, M_1 |
| Sample type |
SRA |
| |
|
| Source name |
Primary cells
|
| Organism |
Oryctolagus cuniculus |
| Characteristics |
cell type: Parathyroid cell
genotype: IRI
treatment: none
time: 6H-6H
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using RNA Quick Purification kit (ESscience). 1.1ug of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using TruePrep RNA Library Prep Kit for Illumina (Vazyme)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
CLC Genomics Workbench v11.0.1
Sequence reads were trimmed for adaptor sequence/low-quality sequence using CLC genomic benchw ork (parameter-Qualitu limit:0.05)
Trimmed sequence reads were mapped to GrCM38/mm10 using CLC genomic benchw ork (parameters-mismath cost:2 insertion cost:3 deletion cost:3 length fraction:0.8 similarity fration:0.8)
Read count extraction and normalization were performed using CLC genomic benchw ork
Supplementary files format and content: tab-delimited text file include FPKM for each sample
|
| |
|
| Submission date |
Aug 04, 2023 |
| Last update date |
Aug 23, 2023 |
| Contact name |
Nianqiu Liu |
| E-mail(s) |
20201650@kmmu.edu.cn
|
| Organization name |
The Third Affiliated Hospital of Kunming Medical University: Yunnan Cancer Center, Kunming, 650000, Yunnan
|
| Department |
Breast Surgery
|
| Street address |
519 Kunzhou Road, Xishan
|
| City |
Kunming |
| State/province |
China/Yunnan |
| ZIP/Postal code |
650118 |
| Country |
China |
| |
|
| Platform ID |
GPL21255 |
| Series (1) |
| GSE240162 |
Gallic Acid Pretreatment Mitigates Parathyroid Ischemia-Reperfusion Injury through Signaling Pathway Modulation |
|
| Relations |
| BioSample |
SAMN36844479 |
| SRA |
SRX21256699 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
