|
| Status |
Public on Aug 02, 2011 |
| Title |
PcDNA3.1-2 |
| Sample type |
RNA |
| |
|
| Source name |
wild-type
|
| Organism |
Mus musculus |
| Characteristics |
cell type: STHdh striatal cells
genotype/variation: wild-type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from striatal cells was extracted using RNAeasy mini kit (Qiagen) with DNase I treatment to eliminate genomic DNA contamination
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled
|
| |
|
| Hybridization protocol |
Samples were hybridized onto the Illumina Sentrix BeadChip Array MouseRef-8 v2 for 18 hours at 58°C (protocol available at http://www.illumina.com/products/mouseref-8_expression_beadchip_kits_v2.ilmn).
|
| Scan protocol |
BeadChip Arrays were scanned using the Illumina BeadArray Reader with default settings.
|
| Description |
replicate 2
|
| Data processing |
Data normalization was performed using GenomeStudio Gene Expression Module v1.0 with quantile normalization.
|
| |
|
| Submission date |
Aug 01, 2011 |
| Last update date |
Aug 02, 2011 |
| Contact name |
Elizabeth A. Thomas |
| Organization name |
The Scripps Research Institute
|
| Department |
Molecular Biology
|
| Street address |
10550 N. Torrey Pines Rd
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE31096 |
Genome-wide identification of Bcl11b gene targets |
|
