|
| Status |
Public on Aug 11, 2023 |
| Title |
Ago-APP in neuroblasts_1 |
| Sample type |
SRA |
| |
|
| Source name |
brain
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: brain
cell type: neuroblasts
genotype: L3 larvae
treatment: Ago-APP
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ago-APP
Truseq small RNA library prep modified according to Kim et al. (2019)
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
NextSeq 550 |
| |
|
| Data processing |
Reads were trimmed at 3' and 5' ends for illumina adaptors and 4 additional nucleotides using Trim-Galore tool (default parameters) provided by useGalaxy.org website
BAM files were generated using "Map with BWA" tool (default parameters) provided by useGalaxy.org website.
micro-RNAs reads were counted in R using the summarizeOverlaps function of GenomicAlignments package
Counts were normalized between samples using the "counts per million" method
Assembly: dm6
Supplementary files format and content: A single Excel file containing normalized data for all samples
|
| |
|
| Submission date |
Aug 10, 2023 |
| Last update date |
Aug 11, 2023 |
| Contact name |
christophe beclin |
| E-mail(s) |
christophe.beclin@univ-amu.fr
|
| Phone |
00 33 491269341
|
| Organization name |
CNRS
|
| Department |
Aix-Marseille université
|
| Lab |
IBDM
|
| Street address |
campus de Luminy, case 907
|
| City |
Marseille |
| State/province |
Bouches du Rhone |
| ZIP/Postal code |
13009 |
| Country |
France |
| |
|
| Platform ID |
GPL22106 |
| Series (1) |
| GSE240560 |
A module of microRNAs cooperatively regulating neurogenesis |
|
| Relations |
| BioSample |
SAMN36933142 |
| SRA |
SRX21326235 |
