|
| Status |
Public on Mar 24, 2024 |
| Title |
4cell_RAGxWT_10, mRNA-seq |
| Sample type |
SRA |
| |
|
| Source name |
4 cell embryo
|
| Organism |
Mus musculus |
| Characteristics |
tissue: 4 cell embryo
genotype: RAG KO x WT
environment: RAGxWT_embryo
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Single embryos, sired by control, germ-free or Rag2−/− sperm or eggs from control or GF mice were collected for single-embryo/egg RNA-sequencing. Embryos were transferred to a 96-well plate and fresh 1 × TCL buffer with 1% β-mercaptoethanol was added.
Nextera XT kit (Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 1000 |
| |
|
| Description |
data_S1_RNAseq_read_cpm.xlsx
|
| Data processing |
Reads were mapped using RSEM and normalized to transcripts per million (tpm).
Assembly: mm10
Supplementary files format and content: xlsx spreadsheet with one tab per tissue type/comparison
|
| |
|
| Submission date |
Aug 14, 2023 |
| Last update date |
Mar 24, 2024 |
| Contact name |
Natalie Alyce Trigg |
| E-mail(s) |
natalie.trigg@uon.edu.au
|
| Phone |
0249212043
|
| Organization name |
Children's Hospital of Philadelphia
|
| Lab |
Conine Lab
|
| Street address |
421 Curie Blvd
|
| City |
Philadelphia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL32159 |
| Series (1) |
| GSE240797 |
The microbiota and T cells non-genetically modulate inherited phenotypes transgenerationally |
|
| Relations |
| BioSample |
SAMN36983375 |
| SRA |
SRX21361345 |
