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Status |
Public on Dec 05, 2023 |
Title |
Male Hippocampus_MREmx1-cre_rep1 |
Sample type |
RNA |
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Source name |
hippocampus, MREmx1-cre
|
Organism |
Mus musculus |
Characteristics |
gender: male strain: C57BL/6 genotype: MREmx1-cre tissue: hippocampus
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA for microarray analysis was isolated from the whole hippocampus of adult male and female dflox control and GREmx1-cre, MREmx1-cre, and GRMREmx1-cre mice using the RNeasy Mini Kit (Qiagen) and RNase-Free DNase Kit (Qiagen).
|
Label |
Cy3
|
Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
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|
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Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
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Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
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Description |
Gene expression from male hippocampus, MREmx1-cre
|
Data processing |
Data was obtained using the Agilent Feature Extraction software (v12.0), using the 1-color defaults for all parameters.
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Submission date |
Aug 15, 2023 |
Last update date |
Dec 05, 2023 |
Contact name |
NIEHS Microarray Core |
E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
Organization name |
NIEHS
|
Department |
DIR
|
Lab |
Microarray Core
|
Street address |
111 T.W. Alexander Drive
|
City |
RTP |
State/province |
NC |
ZIP/Postal code |
27709 |
Country |
USA |
|
|
Platform ID |
GPL7202 |
Series (1) |
GSE240873 |
Glucocorticoid and Mineralocorticoid Receptor Signaling have Sex-dependent and Independent Effects in Mouse Hippocampus |
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