|
| Status |
Public on Mar 08, 2024 |
| Title |
ESC_WT-1 (RNA-seq rep-1) |
| Sample type |
SRA |
| |
|
| Source name |
HBG3 ES cell line
|
| Organism |
Mus musculus |
| Characteristics |
cell line: HBG3 ES cell line
cell type: Embryonic stem cell (ESC)
genotype: WT
treatment: Stem cell maintenance
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
not provided
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Reads were aligned to mm10 using STAR (STAR-2.5.4b --alignEndsType EndToEnd --outFilterMultimapNmax 1 --outFilterMismatchNmax 4. TPM was generated using Kallisto. FeatureCounts was used to generate read counts. Differential gene expression analysis were performed using R package Deseq2.
Assembly: mm10
Supplementary files format and content: txt. gene expression
|
| |
|
| Submission date |
Aug 21, 2023 |
| Last update date |
Mar 08, 2024 |
| Contact name |
Chia-ho Lin |
| E-mail(s) |
CHIAHOLI@g.UCLA.EDU
|
| Organization name |
UCLA
|
| Lab |
BLACK LAB
|
| Street address |
6577 MRL. 675 CHARLES E YOUNG DR. SOUTH
|
| City |
LOS ANGELES |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE241377 |
Alternative splicing of a chromatin modifier alters the transcriptional regulatory programs of embryonic stem cell maintenance and neuronal differentiation [RNA-seq] |
| GSE241378 |
Alternative splicing of a chromatin modifier alters the transcriptional regulatory programs of embryonic stem cell maintenance and neuronal differentiation. |
|
| Relations |
| BioSample |
SAMN37097506 |
| SRA |
SRX21438736 |
