|
| Status |
Public on Nov 10, 2023 |
| Title |
eyeRiboRNA_WT3 |
| Sample type |
SRA |
| |
|
| Source name |
eye discs
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: eye discs
genotype: ey-GAL4/+
treatment: flies maintained at 25oC, eye discs with mouth hooks were dissected from late L3 larvae.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted byTRizol following the product manual.
Library was constructed by standard Illumina protocol. Ribo-seq library was generated by NEBNext® Multiplex Small RNA Library Prep Set for Illumina (E7300S, New England BioLabs).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 2000 |
| |
|
| Description |
RNA-seq
|
| Data processing |
For RNA-seq of total RNA samples, fastq files are mapped to fly dm6 genome by Tophat to get bam flies. The bam files are sorted by samtools.v1.9 sort and index. Sorted bam files are used to generate bigwig graph and get raw counts in CDS regions by Htseq. Raw counts are normalized to get RNA expression levels.
Assembly: dm6
Supplementary files format and content: bw
|
| |
|
| Submission date |
Aug 23, 2023 |
| Last update date |
Nov 10, 2023 |
| Contact name |
Weidong Wang |
| E-mail(s) |
wangw@grc.nia.nih.gov
|
| Phone |
4104548418
|
| Organization name |
National Institute of Aging
|
| Lab |
LGG
|
| Street address |
251 Bayview Blvd BRC, Suite 100 Rm10B014
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21224 |
| Country |
USA |
| |
|
| Platform ID |
GPL30203 |
| Series (1) |
| GSE232395 |
Study Nocte's functions in transcription and translation in Drosophila eye discs |
|
| Relations |
| BioSample |
SAMN37125176 |
| SRA |
SRX21467943 |
