|
| Status |
Public on Nov 21, 2023 |
| Title |
SHR_control_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Mandible body (molar region)
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: shr
condition: control
|
| Growth protocol |
Periodontal disease was induced in 10-week-old male Wistar and SHR rats by the bilateral placement of ligatures around the mandibular first molars, maintained for 15 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was carried out using the mirVana™ miRNA Isolation Kit (Thermo Fisher), following the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA samples (250 ng) underwent poly(A) tailing and biotin-based tagging using the FlashTag™ Biotin HSR RNA Labeling Kit (Thermo Fisher), following the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Sample hybridization was conducted for 20 hours at 48°C, followed by washes and staining steps using the GeneAtlas™ Hybridization, Wash, and Stain Kit (Thermo Fisher Scientific), following the manufacturer's instructions.
|
| Scan protocol |
Microarray strips were scanned using the Gene Atlas Imaging Station (Thermo Fisher Scientific).
|
| Description |
MicroRNA expression in the mandibles of SHR rats (control group)
|
| Data processing |
Transcriptome Analysis Console Software version 4.0.2 (Thermo Fisher Scientific) was used for data normalization and detection of expression signals, using the robust multichip analysis (RMA) and detection above background (DABG) algorithms. The same software was used for conducting differential expression analysis, employing the limma package with eBayes statistics (criteria: p<0.05 and fold change ≥ 1.5 or ≤ -1.5).
|
| |
|
| Submission date |
Aug 28, 2023 |
| Last update date |
Nov 21, 2023 |
| Contact name |
Victor Gustavo Balera Brito |
| E-mail(s) |
vgb.brito@unesp.br
|
| Organization name |
São Paulo State University
|
| Department |
Basic sciences
|
| Street address |
Marechal Rondon, km 527/8
|
| City |
Araçatuba |
| State/province |
SP |
| ZIP/Postal code |
16066-840 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE241787 |
MicroRNA expression in mandibles of Wistar and Spontaneously Hypertensive rats with periodontal disease |
|
