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Status |
Public on Sep 05, 2023 |
Title |
HiC, -met, rep2 |
Sample type |
SRA |
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Source name |
yMD291
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Organism |
Saccharomyces cerevisiae |
Characteristics |
strain: yMD291 chip antibody: NA condition: NA genotype: MATa, his3-11,15, leu2-3,112, trp1-1, ura3-1, can1-100, ade2-1, tor1-1, fpr::NAT, bar1, HIS3::REV1pr-tetR-mCherry-REV1pr-LacI-GFP, ADE2::REV1pr-LacI-REV1pr-tetR, Chr15::497047_LacO, Chr15::834487_TetO treatment: methionine depletion
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Growth protocol |
Yeast strains were grown in 200 mL synthetic media without methionine to OD660 = 0.3.
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Extracted molecule |
genomic DNA |
Extraction protocol |
Standard Hi-C protocol was used for the preparation of Hi-C samples Hi-C libraries were prepared with NEBNext Ultra II DNA Library Prep Kit. Next generation sequencing was performed on NextSeq 2000.
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Library strategy |
Hi-C |
Library source |
genomic |
Library selection |
other |
Instrument model |
NextSeq 2000 |
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Data processing |
150 bp paired-end reads were aligned to S. cerevisiae genome (sacCer3) and analyzed with HiC-pro. Assembly: sacCer3 Supplementary files format and content: BED file includes Hi-C bins Supplementary files format and content: matrix file of Hi-C dataset
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Submission date |
Sep 05, 2023 |
Last update date |
Sep 07, 2023 |
Contact name |
Lu Bai |
E-mail(s) |
lub15@psu.edu
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Organization name |
Penn State University
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Department |
BMB
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Lab |
Bai
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Street address |
406 SOUTH FREAR, UNIVERSITY PARK
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City |
UNIVERSITY PARK |
State/province |
PENNSYLVANIA |
ZIP/Postal code |
16802 |
Country |
USA |
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Platform ID |
GPL31112 |
Series (2) |
GSE242396 |
DNA Methylation-Based High-Resolution Mapping of Long-Distance Chromosomal Interactions in Nucleosome-Depleted Regions (HiC) |
GSE242400 |
DNA Methylation-Based High-Resolution Mapping of Long-Distance Chromosomal Interactions in Nucleosome-Depleted Regions |
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Relations |
BioSample |
SAMN37286221 |
SRA |
SRX21638549 |
Supplementary file |
Size |
Download |
File type/resource |
GSM7762059_HiC_S8_10000_iced.matrix.gz |
3.0 Mb |
(ftp)(http) |
MATRIX |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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