|
| Status |
Public on Aug 02, 2024 |
| Title |
Msn2_1_24h |
| Sample type |
SRA |
| |
|
| Source name |
Fungal hyphae cells
|
| Organism |
Orbilia oligospora |
| Characteristics |
tissue: Fungal hyphae
genotype: delta_Aomsn2
treatment: 24 hours of contact with C. elegans cells
|
| Treatment protocol |
Mycelium was collected and washed three times with 0.1 mphosphate buffer saline (PBS) (pH 6.8-7.2).
|
| Growth protocol |
The WT and ΔAomsn2 strains were cultured in a PDA medium for 7 days. Afterward, 200 C. elegans cells were added to each plate for 24 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After collecting the samples, RNA was extracted with the AxyPrep Multisource RNA Miniprep Kit (Axygen, Jiangsu, China)
The libraries were prepared for sequencing using standard Illumina protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
original provided organism name: Arthrobotrys oligospora
|
| Data processing |
The data from high-throughput sequencing were analyzed using the OmicShare tools, a free online platform for data analysis (www.majorbio.com).
Assembly: https://www.ncbi.nlm.nih.gov/assembly/GCF_000225545.1
Supplementary files format and content: Expression analysis table include TPM values for each sample
Supplementary files format and content: Matrix table with raw gene counts for every gene and every sample
|
| |
|
| Submission date |
Sep 06, 2023 |
| Last update date |
Aug 02, 2024 |
| Contact name |
Liu Qianqian |
| E-mail(s) |
liuqianqian@mail.ynu.edu.cn
|
| Organization name |
Yunnan University
|
| Street address |
No.2 Cuihu North Road, Wuhua District
|
| City |
Kunming City, Yunnan Province |
| ZIP/Postal code |
650032 |
| Country |
China |
| |
|
| Platform ID |
GPL31134 |
| Series (1) |
| GSE242457 |
Transcriptome analysis of Aomsn2 in nematode-trapping fungus Arthrobotrys oligospora |
|
| Relations |
| BioSample |
SAMN37300232 |
| SRA |
SRX21646547 |
