|
Status |
Public on Sep 17, 2023 |
Title |
D1491T19_RNA-seq_p50_AH_rep2 |
Sample type |
SRA |
|
|
Source name |
full embryo
|
Organism |
Bombyx mori |
Characteristics |
tissue: full embryo genotype: p50
|
Growth protocol |
~200 p50 diapaused eggs harvest after release 24h at 25°C
|
Extracted molecule |
total RNA |
Extraction protocol |
Eggs or adult head and thorax were placed in TRIzol™ Reagent from ThermoFisher Scientific before mechanically broken with a piston pellet to allow total RNA extraction. Sequencing libraries have been obtained using the Illumina stranded mRNA prep ligation protocol.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
RNA-seq RNA-seq_p50_PD-D2-24h_AH_TPM.xlsx
|
Data processing |
Library were sequenced on Novaseq. Adapter sequences were trimmedfrom reads using trimgalore. RNA-seq reads were aligned to our custom Bmori_v4 genome assembly using STAR_2.7.8a. Assembly: Bmori_v4 Supplementary files format and content: Excel table file Supplementary files format and content: bigWig
|
|
|
Submission date |
Sep 08, 2023 |
Last update date |
Sep 17, 2023 |
Contact name |
Héloïse Muller |
E-mail(s) |
heloise.muller@curie.fr
|
Organization name |
Institut Curie
|
Lab |
UMR3664
|
Street address |
26 rue d'Ulm
|
City |
Paris |
ZIP/Postal code |
75005 |
Country |
France |
|
|
Platform ID |
GPL29210 |
Series (2) |
GSE228398 |
Unique territorial and sub-chromosomal organization revealed in the holocentric moth Bombyx mori [RNA-seq] |
GSE228401 |
Unique territorial and sub-chromosomal organization revealed in the holocentric moth Bombyx mori |
|
Relations |
BioSample |
SAMN37333237 |
SRA |
SRX21708147 |