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GEO accession:
Sample GSM77715
Query DataSets for GSM77715
Status
Public on Dec 31, 2005
Title
CuSO4 low replicate 1
Sample type
RNA
Source name
Daphnia magna neonate
Organism
Daphnia magna
Characteristics
Wild type, female, neonate, whole body
Treatment protocol
Exposed to 5 ug/l of copper sulphate for 24 h.
Extracted molecule
total RNA
Extraction protocol
Approximately 10 neonates were lysed in RLT buffer (Qiagen).Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label
Cy3
Label protocol
Approximately 0.5ug of total RNA was processed to produce cRNA targets.
Scan protocol
standard GenePix procedures
Description
Wild type neonate (<24h)
Data processing
GeneSpring version7.2
Submission date
Oct 12, 2005
Last update date
Oct 13, 2005
Contact name
Hajime Watanabe
E-mail(s)
watanabe@nibb.ac.jp
Phone
81-564-59-5237
Fax
81-564-59-5236
Organization name
National Institutes of Natural Sciences
Department
Center for Integrative Bioscience
Street address
5-1, Higashiyama
City
Okazaki
State/province
Aichi
ZIP/Postal code
444-8787
Country
Japan
Platform ID
GPL2888
Series (1)
GSE3445
Daphnia magna DNA microarray for the evaluation of toxicity of environmental chemicals
Data table header descriptions
ID_REF
VALUE
GeneSpring-calculated Signal intensity
Data table
ID_REF
VALUE
111
74
112
302
113
3655
115
16
116
30
121
130
122
341
123
218
125
262
126
6817
131
5101
132
119
133
8
135
61
136
103
141
32
142
212
143
122
145
137
146
58
Total number of rows:
178
Table truncated, full table size
1 Kbytes
.
Supplementary data files not provided
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