|
| Status |
Public on Apr 30, 2024 |
| Title |
CHD015-17 |
| Sample type |
genomic |
| |
|
| Source name |
Case#2_t(8;21)
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Unsorted Bone Marrow Cells
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA were extracted from bone marrow cells of patients using All Prep DNA/RNA Mini Kit (Qiagen). DNA quality and quantity was assessed using a Nanodrop Spectrophotometer, Bioanalyzer and/or Tapestation visualization (Agilent 2100 Bioanalyzer) and agarose gel electrophoresis
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, ligated, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000 7G
|
| Description |
CHD015-17
|
| Data processing |
Protocol was supported by Affymetrix GeneChip Command Console (AGCC) software. Analysis was performed through Affymetrix Chromosome Analysis 4.0 (ChAS) software with filters set at 200 Kb for Copy Number Alterations (CNAs) and 10 Mb for copy neutral Loss Of Heterozygosity (cnLOH). Our data refer to NetAffx Build 32.3 (hg19) database
|
| |
|
| Submission date |
Sep 13, 2023 |
| Last update date |
Apr 30, 2024 |
| Contact name |
Cristina Mecucci |
| E-mail(s) |
cristina.mecucci@unipg.it
|
| Phone |
+39 075 5783808
|
| Organization name |
University of Perugia
|
| Department |
Department of Medicine, Hematology and Bone Marrow Transplantation Unit
|
| Lab |
Laboratory of Cytogenetics and Molecular Genetics
|
| Street address |
Piazzale Menghini n.9
|
| City |
Perugia |
| ZIP/Postal code |
06132 |
| Country |
Italy |
| |
|
| Platform ID |
GPL16131 |
| Series (2) |
| GSE243055 |
A novel t(X;21) (p11.4;q22.12) translocation adds to the role of BCOR and RUNX1 in Myelodysplastic Syndromes/Acute Myeloid Leukemias [Copy number analysis] |
| GSE243056 |
A novel t(X;21) (p11.4;q22.12) translocation adds to the role of BCOR and RUNX1 in Myelodysplastic Syndromes/Acute Myeloid Leukemias |
|
