NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM7792382 Query DataSets for GSM7792382
Status Public on Oct 28, 2023
Title Plasma EVPs Young-adult 10
Sample type RNA
 
Source name Plasma_Young-Total
Organism Rattus norvegicus
Characteristics tissue: plasma extracellular vesicles and particles
age: 3-month-old
Sex: Male
Extracted molecule total RNA
Extraction protocol extracellular vesicles and particles were isolated from plasm using Exoquick precipitation reagent. Total RNA was extracted using mirVana RNA isolation kits. Total RNA was amplified with the complete Seramir Exosome RNA amplification kit.
Label Biotin
Label protocol 1,000 ng of RNA was labeled using Affymetrix FlashTag Biotin HSR RNA labeling kit.
 
Hybridization protocol Following labeling RNA was hybridized to affymetrix miRNA 4.0 GeneChips for 16 hours and washed and stained on the GeneChip Fluidics Station 450 using protocol FS450_0002.
Scan protocol GeneChips were scanned on the Affymetrix GeneChip Scanner 3000
Description Circulating plasma Extracellular vesicles and particles_Young adult
miRNA expression data
Data processing Resulting data were normalized in Affymetrix Expression Console software using RMA-DABG and exported into Partek Genomics Suite version 6.6 for analysis.
 
Submission date Sep 20, 2023
Last update date Oct 28, 2023
Contact name Laura Reck Cechinel
E-mail(s) lreck@childrensnational.org
Organization name Children's National Medical Center
Street address 7144 13th Pl NW
City Washington
State/province District of Columbia
ZIP/Postal code 20012
Country USA
 
Platform ID GPL21572
Series (1)
GSE243656 Circulating total extracellular vesicles cargo are associated with age-related oxidative stress and susceptibility to cardiovascular diseases: exploratory results from microarray data

Data table header descriptions
ID_REF
VALUE RMA-DABG
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
20500924 1.3347 0.294063
20500925 0.943296 0.978959
20500928 0.974517 0.959988
20500929 1.58246 0.34251
20500932 0.755812 0.992864
20500933 1.64269 0.433853
20500934 10.6721 8.72176e-16
20500935 1.88163 0.141925
20500938 0.838862 0.931851
20500939 1.05162 0.621007
20500944 2.13977 0.0581579
20500945 1.32061 0.761476
20500946 3.20988 0.00104136
20500947 1.55829 0.296017
20500948 7.02221 4.41998e-06
20500949 7.82141 2.22934e-09
20500950 2.27619 0.134468
20500953 0.927648 0.810917
20500954 0.818561 0.763553
20500957 1.30177 0.638955

Total number of rows: 1277

Table truncated, full table size 32 Kbytes.




Supplementary file Size Download File type/resource
GSM7792382_10_miRNA-4_0_.CEL.gz 776.6 Kb (ftp)(http) CEL
GSM7792382_10_miRNA-4_0_.rma-dabg.chp.gz 18.6 Kb (ftp)(http) CHP

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.