|
Status |
Public on Oct 28, 2023 |
Title |
Plasma EVPs Young-adult 10 |
Sample type |
RNA |
|
|
Source name |
Plasma_Young-Total
|
Organism |
Rattus norvegicus |
Characteristics |
tissue: plasma extracellular vesicles and particles age: 3-month-old Sex: Male
|
Extracted molecule |
total RNA |
Extraction protocol |
extracellular vesicles and particles were isolated from plasm using Exoquick precipitation reagent. Total RNA was extracted using mirVana RNA isolation kits. Total RNA was amplified with the complete Seramir Exosome RNA amplification kit.
|
Label |
Biotin
|
Label protocol |
1,000 ng of RNA was labeled using Affymetrix FlashTag Biotin HSR RNA labeling kit.
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|
|
Hybridization protocol |
Following labeling RNA was hybridized to affymetrix miRNA 4.0 GeneChips for 16 hours and washed and stained on the GeneChip Fluidics Station 450 using protocol FS450_0002.
|
Scan protocol |
GeneChips were scanned on the Affymetrix GeneChip Scanner 3000
|
Description |
Circulating plasma Extracellular vesicles and particles_Young adult miRNA expression data
|
Data processing |
Resulting data were normalized in Affymetrix Expression Console software using RMA-DABG and exported into Partek Genomics Suite version 6.6 for analysis.
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Submission date |
Sep 20, 2023 |
Last update date |
Oct 28, 2023 |
Contact name |
Laura Reck Cechinel |
E-mail(s) |
lreck@childrensnational.org
|
Organization name |
Children's National Medical Center
|
Street address |
7144 13th Pl NW
|
City |
Washington |
State/province |
District of Columbia |
ZIP/Postal code |
20012 |
Country |
USA |
|
|
Platform ID |
GPL21572 |
Series (1) |
GSE243656 |
Circulating total extracellular vesicles cargo are associated with age-related oxidative stress and susceptibility to cardiovascular diseases: exploratory results from microarray data |
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