|
| Status |
Public on Sep 25, 2023 |
| Title |
A-B-plasmid-library-Gecko |
| Sample type |
SRA |
| |
|
| Source name |
not cell (plasmid)
|
| Organism |
synthetic construct |
| Characteristics |
cell type: not cell (plasmid)
|
| Treatment protocol |
Cells were treated by DMSO or by 40µM of AZD7648 during 72h.
|
| Growth protocol |
Cells were cultured in RPMI medium 1640 (Sigma Life Technologies, Saint Louis, MO) supplemented with 10% fetal calf serum (Dutscher, France) at 37 °C with 5% CO2
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using the DNeasy Blood and Tissue kit (Qiagen ID: 69504) following the manufacturer's protocol
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
The entire genomic DNA was sequenced by Nucleus Biotech. SgRNA was amplified, and Next-Generation Sequencing (NGS) was performed on the Illumina NextSeq platform, targeting 60 million reads.
Supplementary files format and content: tab-delimited text file with CrispR cloud fold change results
Supplementary files format and content: Excel file with RIGER fold change results
Supplementary files format and content: Excel file with RIGER calculations
Supplementary files format and content: tab-delimited text file with Mageck fold change results
|
| |
|
| Submission date |
Sep 21, 2023 |
| Last update date |
Sep 25, 2023 |
| Contact name |
Amandine Crombe |
| E-mail(s) |
crombeamandine2@gmail.com
|
| Organization name |
Institut Bergonié
|
| Street address |
229 cours de l'Argonne
|
| City |
Bordeaux |
| ZIP/Postal code |
33000 |
| Country |
France |
| |
|
| Platform ID |
GPL19424 |
| Series (1) |
| GSE243758 |
Selective DNA-PK inhibition enhances chemotherapy and ionizing radiation activity in soft-tissue sarcomas |
|
| Relations |
| BioSample |
SAMN37503075 |
| SRA |
SRX21853572 |
