|
| Status |
Public on Jun 01, 2024 |
| Title |
hypothalamus, 60 day, M2-5 |
| Sample type |
SRA |
| |
|
| Source name |
hypothalamus
|
| Organism |
Capra hircus |
| Characteristics |
tissue: hypothalamus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent.
A total of 1 µg total RNA per sample was used as input material for the lncRNA library preparation. Strand-specific libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Clean data (clean reads) were obtained by trimming reads containing adapter, ploy-N or with low quality from raw data
Index of the reference genome was built using hisat2-v2.0.5 and paired-end clean reads were mapped to the reference genome.
Stringtie-1.3.3b was used to count the read numbers mapped to each gene.
Assembly: ARS1.2
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample.
|
| |
|
| Submission date |
Sep 25, 2023 |
| Last update date |
Jun 01, 2024 |
| Contact name |
Qing Li |
| E-mail(s) |
2022010086@sdau.edu.cn
|
| Phone |
+8618853852560
|
| Organization name |
College of Animal Science and Veterinary Medicine
|
| Street address |
Tai’an, Shandong Province, P.R. China
|
| City |
Tai'an |
| ZIP/Postal code |
271018 |
| Country |
China |
| |
|
| Platform ID |
GPL27106 |
| Series (1) |
| GSE244004 |
Transcriptome analysis revealed the characteristics and functions of long non-coding RNAs in the hypothalamus during sexual maturation in goats |
|
| Relations |
| BioSample |
SAMN37537020 |
| SRA |
SRX21882245 |
