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Sample GSM7808526 Query DataSets for GSM7808526
Status Public on Feb 26, 2024
Title CUTLL1 CT rep3
Sample type SRA
 
Source name CUTLL1
Organism Homo sapiens
Characteristics cell line: CUTLL1
cell type: T-ALL
genotype: t(7;9)(q34;q34) translocation
treatment: CT shRNA
Treatment protocol CUTLL1 cells were transduced with lentivirus containing pLKO CT/KD shRNA. After 48 hours, cells were treated with 1ug/ml puromycin for 5 days.
Growth protocol complete RPMI + 20% FBS
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Qiagen RNeasy kit
NuGEN Trio RNA-seq library preparation kit
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
 
Data processing Alignment STAR version=2.7.9a -sjdbOverhang 100
Read counts htseq 0.11.4
Differential Expression Analysis: DESeq2_1.38.3
Assembly: GRCh38 Gencode release 39
Supplementary files format and content: HTSeq counts file tab delimeted
 
Submission date Sep 27, 2023
Last update date Feb 26, 2024
Contact name Stanley Adoro
E-mail(s) stanley.adoro@nih.gov
Organization name NCI
Department CCR
Lab EIB
Street address Center Dr
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL20795
Series (2)
GSE244198 CHMP5-Mediated Transcriptional Regulation of T-Cell Acute Lymphoblastic Leukemia (Human RNAseq)
GSE244200 CHMP5-Mediated Transcriptional Regulation of T-Cell Acute Lymphoblastic Leukemia
Relations
BioSample SAMN37568633
SRA SRX21909355

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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