|
Status |
Public on Feb 26, 2024 |
Title |
CUTLL1 CT rep3 |
Sample type |
SRA |
|
|
Source name |
CUTLL1
|
Organism |
Homo sapiens |
Characteristics |
cell line: CUTLL1 cell type: T-ALL genotype: t(7;9)(q34;q34) translocation treatment: CT shRNA
|
Treatment protocol |
CUTLL1 cells were transduced with lentivirus containing pLKO CT/KD shRNA. After 48 hours, cells were treated with 1ug/ml puromycin for 5 days.
|
Growth protocol |
complete RPMI + 20% FBS
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using the Qiagen RNeasy kit NuGEN Trio RNA-seq library preparation kit
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
HiSeq X Ten |
|
|
Data processing |
Alignment STAR version=2.7.9a -sjdbOverhang 100 Read counts htseq 0.11.4 Differential Expression Analysis: DESeq2_1.38.3 Assembly: GRCh38 Gencode release 39 Supplementary files format and content: HTSeq counts file tab delimeted
|
|
|
Submission date |
Sep 27, 2023 |
Last update date |
Feb 26, 2024 |
Contact name |
Stanley Adoro |
E-mail(s) |
stanley.adoro@nih.gov
|
Organization name |
NCI
|
Department |
CCR
|
Lab |
EIB
|
Street address |
Center Dr
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL20795 |
Series (2) |
GSE244198 |
CHMP5-Mediated Transcriptional Regulation of T-Cell Acute Lymphoblastic Leukemia (Human RNAseq) |
GSE244200 |
CHMP5-Mediated Transcriptional Regulation of T-Cell Acute Lymphoblastic Leukemia |
|
Relations |
BioSample |
SAMN37568633 |
SRA |
SRX21909355 |