|
Status |
Public on Apr 16, 2024 |
Title |
W0K271 |
Sample type |
SRA |
|
|
Source name |
JJN3 human multiple myeloma cell line
|
Organism |
Homo sapiens |
Characteristics |
treatment: DMSO antibody: CST D5E4 #8173 (H3K27ac) cell line: JJN3 cell type: multiple myeloma
|
Treatment protocol |
Cells were treated with DMSO or WNK463 (5μM) for 4 h
|
Growth protocol |
JJN3 cells were cultured in 40% DMEM 40% IMDM, and 20% FBS in 37°C supplemented with 10% CO2
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Protein-DNA complexes were isolated by antibody Libraries were prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) (Cells Signaling Technology) and Multiplex Oligos for Illumina Systems (Single Index Primers) (ChIP-seq, CUT&RUN) (Cells Signaling Technology) following the manufacturer’s protocol
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Reads mapped to hg38 with BWA Duplicates removed with PICARD Bigwig files generated with deeptools Assembly: hg38 Supplementary files format and content: bigwig
|
|
|
Submission date |
Oct 04, 2023 |
Last update date |
Apr 16, 2024 |
Contact name |
Rui Li |
Organization name |
University of Massachusetts Medical School
|
Department |
MCCB
|
Street address |
364 Plantation Street, LRB
|
City |
Worcester |
State/province |
Massachusetts |
ZIP/Postal code |
01605 |
Country |
USA |
|
|
Platform ID |
GPL24676 |
Series (2) |
GSE244630 |
Global mapping of H3K27ac and RPB1 occupancy upon WNK463 treatment |
GSE244670 |
WNK463 effect on JJN3 cells |
|
Relations |
BioSample |
SAMN37686128 |
SRA |
SRX21991760 |