|
| Status |
Public on Apr 16, 2024 |
| Title |
W0K271 |
| Sample type |
SRA |
| |
|
| Source name |
JJN3 human multiple myeloma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: DMSO
antibody: CST D5E4 #8173 (H3K27ac)
cell line: JJN3
cell type: multiple myeloma
|
| Treatment protocol |
Cells were treated with DMSO or WNK463 (5μM) for 4 h
|
| Growth protocol |
JJN3 cells were cultured in 40% DMEM 40% IMDM, and 20% FBS in 37°C supplemented with 10% CO2
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Protein-DNA complexes were isolated by antibody
Libraries were prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) (Cells Signaling Technology) and Multiplex Oligos for Illumina Systems (Single Index Primers) (ChIP-seq, CUT&RUN) (Cells Signaling Technology) following the manufacturer’s protocol
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Reads mapped to hg38 with BWA
Duplicates removed with PICARD
Bigwig files generated with deeptools
Assembly: hg38
Supplementary files format and content: bigwig
|
| |
|
| Submission date |
Oct 04, 2023 |
| Last update date |
Apr 16, 2024 |
| Contact name |
Rui Li |
| Organization name |
University of Massachusetts Medical School
|
| Department |
MCCB
|
| Street address |
364 Plantation Street, LRB
|
| City |
Worcester |
| State/province |
Massachusetts |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE244630 |
Global mapping of H3K27ac and RPB1 occupancy upon WNK463 treatment |
| GSE244670 |
WNK463 effect on JJN3 cells |
|
| Relations |
| BioSample |
SAMN37686128 |
| SRA |
SRX21991760 |
