|
Status |
Public on Jul 11, 2013 |
Title |
olive oil 4d 101253-35 |
Sample type |
RNA |
|
|
Source name |
CsA v 4d
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 gender: male age: 10 weeks weight: 21-27 gram tissue: liver treatment: olive oil time: 4d
|
Treatment protocol |
Mice treated for 1, 4, 11 days with vehicle (olive oil) or 3, 8.9, or 26.7 mg/kg BW CsA
|
Extracted molecule |
total RNA |
Extraction protocol |
Upon sacrifice, liver was immediately dissected. Central half of the left lateral lobe was collected in RNA later and kept for a maximum of one week at 4 oC and frozen at -80 oC until use for RNA isolation. RNA was isolated using the RNeasy Mini Kit.
|
Label |
biotin
|
Label protocol |
According to the protocol provided with the Affymetrix 3'IVT Express Labeling Kit (#901229)
|
|
|
Hybridization protocol |
According to the Affymetrix Hybridization, Wash and Stain Kit (#901530)
|
Scan protocol |
According to Affymetrix protocols
|
Description |
quality control: approved
|
Data processing |
CEL files generated using Affymetrix GeneChip Command Console (v3.0) software were normalized using RMA and the MBNI custon CDF (http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/CDF/). Quality control consisted of the various RMA QC parameters as well as visual inspection of residual plots created in RMA express. Two samples did not meet these quality criteria.
|
|
|
Submission date |
Aug 19, 2011 |
Last update date |
Jul 11, 2013 |
Contact name |
Anne Susan Kienhuis |
E-mail(s) |
anne.kienhuis@gmail.com
|
Organization name |
RIVM
|
Street address |
A. van Leeuwenhoeklaan 9
|
City |
Bilthoven |
ZIP/Postal code |
3720 BA |
Country |
Netherlands |
|
|
Platform ID |
GPL14178 |
Series (1) |
GSE31540 |
Gene expression profiles for onset and progression of Cyclosporin A-induced cholestasis in C57BL/6 mice |
|