|
| Status |
Public on Jul 11, 2013 |
| Title |
26.7 mg/kg BW Csa 11d 101253-42 |
| Sample type |
RNA |
| |
|
| Source name |
CsA high 11d
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: male
age: 10 weeks
weight: 21-27 gram
tissue: liver
treatment: 26.7 mg/kg BW Csa
time: 11d
|
| Treatment protocol |
Mice treated for 1, 4, 11 days with vehicle (olive oil) or 3, 8.9, or 26.7 mg/kg BW CsA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Upon sacrifice, liver was immediately dissected. Central half of the left lateral lobe was collected in RNA later and kept for a maximum of one week at 4 oC and frozen at -80 oC until use for RNA isolation. RNA was isolated using the RNeasy Mini Kit.
|
| Label |
biotin
|
| Label protocol |
According to the protocol provided with the Affymetrix 3'IVT Express Labeling Kit (#901229)
|
| |
|
| Hybridization protocol |
According to the Affymetrix Hybridization, Wash and Stain Kit (#901530)
|
| Scan protocol |
According to Affymetrix protocols
|
| Description |
quality control: approved
|
| Data processing |
CEL files generated using Affymetrix GeneChip Command Console (v3.0) software were normalized using RMA and the MBNI custon CDF (http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/CDF/). Quality control consisted of the various RMA QC parameters as well as visual inspection of residual plots created in RMA express. Two samples did not meet these quality criteria.
|
| |
|
| Submission date |
Aug 19, 2011 |
| Last update date |
Jul 11, 2013 |
| Contact name |
Anne Susan Kienhuis |
| E-mail(s) |
anne.kienhuis@gmail.com
|
| Organization name |
RIVM
|
| Street address |
A. van Leeuwenhoeklaan 9
|
| City |
Bilthoven |
| ZIP/Postal code |
3720 BA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL14178 |
| Series (1) |
| GSE31540 |
Gene expression profiles for onset and progression of Cyclosporin A-induced cholestasis in C57BL/6 mice |
|
