|
| Status |
Public on Mar 22, 2013 |
| Title |
Expt. db/db islet Replicate 4 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Control 9 Weeks
|
| Organism |
Mus musculus |
| Characteristics |
age: 9 weeks
tissue: pancreatic islet
genotype: db/+
genetic background: C57BLKS/J
|
| Growth protocol |
Pancreatic islets were isolated from the db/db and the lean control (db/+) mice at 9-week of age. The db/db mice are obese, mildly hyperglycemic and severely hyperinsulinemic at 6 week, representing the compensating stage. Whereas at 9 week of age, the db/db mice become severely hyperglycemic and only modestly hyperinsulinemic (significantly declined from the 6-wk levels), indicating the de-compensation of beta-cell to the insulin resistant state.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
100 ng-input two-round RT/IVT RNA amplification protocol
|
| |
|
| Channel 2 |
| Source name |
db/db 9 week #4
|
| Organism |
Mus musculus |
| Characteristics |
genotype: db/db
age: 9 weeks
tissue: pancreatic islet
genetic background: C57BLKS/J
|
| Growth protocol |
Pancreatic islets were isolated from the db/db and the lean control (db/+) mice at 9-week of age. The db/db mice are obese, mildly hyperglycemic and severely hyperinsulinemic at 6 week, representing the compensating stage. Whereas at 9 week of age, the db/db mice become severely hyperglycemic and only modestly hyperinsulinemic (significantly declined from the 6-wk levels), indicating the de-compensation of beta-cell to the insulin resistant state.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
100 ng-input two-round RT/IVT RNA amplification protocol
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner.
Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
|
| Description |
Biological replicate 4 of 5 db/db islet
|
| Data processing |
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Aug 22, 2011 |
| Last update date |
Mar 22, 2013 |
| Contact name |
I-Ming Wang |
| E-mail(s) |
i_ming_wang@merck.com
|
| Phone |
215-652-1287
|
| Organization name |
Merck Research Lab
|
| Department |
Genetics and Pharmacogenomics
|
| Lab |
Discovery Pharmacogenomics
|
| Street address |
770 Sumneytown Pike
|
| City |
West Point |
| State/province |
PA |
| ZIP/Postal code |
19486 |
| Country |
USA |
| |
|
| Platform ID |
GPL8591 |
| Series (2) |
| GSE31559 |
Various mice and rat tissues subjected to various treatments |
| GSE31953 |
Profiling pancreatic islets from obese db/db and lean control db/+ mice |
|
