|
| Status |
Public on Apr 01, 2024 |
| Title |
fibroblast line 1 |
| Sample type |
SRA |
| |
|
| Source name |
fibroblast_1
|
| Organism |
Mus minutoides |
| Characteristics |
cell line: fibroblast_1
cell type: fibroblast
treatment: no reprograming
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Maxwell RSC simply RNA Cell Kit (Promega).
Library preparation for RNA-seq was performed using the NEBNext Ultra Ⅱ RNA Library Prep Kit for Illumina (NEB) following manifacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
CLC Genomics Workbench 22.0.1
Sequence reads were trimmed using CLC Genomics Workbench 22.0.1 (Quality limit = 0.05).
Trimmed sequence reads were mapped to Mus musculus GRCm38 release-94 using CLC Genomics Workbench 22.0.1.
Read count extacrion and normalization were performed using CLC Genomics Workbench 22.0.1.
|
| |
|
| Submission date |
Oct 11, 2023 |
| Last update date |
Apr 01, 2024 |
| Contact name |
Sumito Matsuya |
| E-mail(s) |
matsuyas@vet.kagoshima-u.ac.jp
|
| Organization name |
Kagoshima university
|
| Department |
Joint Faculty of Veterinary Medicine
|
| Street address |
1-21-24 Korimoto
|
| City |
Kagoshima |
| ZIP/Postal code |
890-0065 |
| Country |
Japan |
| |
|
| Platform ID |
GPL33840 |
| Series (1) |
| GSE245095 |
The comparison of iPSCs and fibroblasts in Mus minutoides |
|
| Relations |
| BioSample |
SAMN37778766 |
| SRA |
SRX22066675 |
