|
| Status |
Public on Jul 09, 2024 |
| Title |
KIT+ cells, E12 FL, scRNAseq |
| Sample type |
SRA |
| |
|
| Source name |
fetal liver
|
| Organism |
Mus musculus |
| Characteristics |
tissue: fetal liver
cell type: KIT-expressing cells
strain: C57BL/6
age: E12
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The fetal liver isolated from E12 mouse embryos was mechanically dissociated with a 23G needle. Those obtained cells were stained with KIT antibody and sorted using an SH800 cell sorter (SONY). Sorted KIT-expressing cells were resuspended in 0.04% BSA/PBS.
Library construction was performed by using the Chromium Single Cell 3' v3.1 kit (10x Genomics).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
The fastq files were aligned, filtered, and counted by the cellranger count (v5.0.0) (10x Genomics).
Assembly: mm10 (GRCm38)
Supplementary files format and content: tab-separated value files and matrix files
|
| |
|
| Submission date |
Oct 16, 2023 |
| Last update date |
Jul 09, 2024 |
| Contact name |
Saori Morino-Koga |
| E-mail(s) |
kogas@kumamoto-u.ac.jp
|
| Organization name |
Kumamoto University
|
| Department |
Institute of Molecular Embryology and Genetics
|
| Lab |
Cell Differentiation
|
| Street address |
2-2-1 Honjo, Chuo-ku
|
| City |
Kumamoto |
| State/province |
Kumamoto |
| ZIP/Postal code |
860-0811 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21273 |
| Series (1) |
| GSE245429 |
KIT-expressing cells in the mouse fetal liver |
|
| Relations |
| BioSample |
SAMN37830788 |
| SRA |
SRX22099615 |
