|
| Status |
Public on Oct 24, 2023 |
| Title |
HCC without metastasis sample 2 |
| Sample type |
RNA |
| |
|
| Source name |
HCC without metastasis sample
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: HCC
tissue type: HCC without metastasis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Trizol/Chloroform, and then purified with magnetic beads of Agencourt Ampure (APN 000132, Beckman Coulter).
|
| Label |
Biotin
|
| Label protocol |
A total of 500 ng RNA was used for a double-round of cDNA synthesis. After fragmentation of 2nd-cycle single-stranded cDNA (ss-cDNA), sample was labeled with biotin by terminal deoxynucleotidyl transferase (TdT).
|
| |
|
| Hybridization protocol |
Ssample was hybridized to the Affymetrix Human Gene 1.0ST Array for 16-18 h at 45°C.
|
| Scan protocol |
The microarrays were washed and stained with Streptavidin Phycoerythrin on the Affymetrix Fluidics Station 450. Microarrays were scanned by using Affymetrix® GeneChip Command Console (AGCC) which installed in GeneChip® Scanner 3000 7G
|
| Description |
HCC tissues didn't exhibited pathological features of metastasis.
Y2_(HTA-2_0).1.sst-rma-gene-full
|
| Data processing |
The data were analyzed with Robust Multichip Analysis (RMA) algorithm using default analysis settings and global scaling as normalization method by Partek® Genomics Suite 6.6. Values presented are log2 RMA signal intensity. Normalized data were further analyzed using one-way ANOVA to screen out the differential expression gene.
|
| |
|
| Submission date |
Oct 17, 2023 |
| Last update date |
Oct 24, 2023 |
| Contact name |
xiangnan teng |
| E-mail(s) |
txn970405@outlook.com
|
| Phone |
1662213831
|
| Organization name |
四川大学华西医院
|
| Street address |
四川大学华西医院
|
| City |
成都 |
| ZIP/Postal code |
610041 |
| Country |
China |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE245587 |
RNA-Binding Protein Trx regulates alternative splicing and promotes metastasis of HCC via interacting with LINC00152 |
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