|
| Status |
Public on May 14, 2024 |
| Title |
Hypothalamus, conjugate 2, rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Hypothalamus
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Hypothalamus
treatment: conjugate 2
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was isolated using the RNeasy mini kit (Qiagen, US) according to the manufacturer’s protocol.
Libraries were prepared using the Universal Plus mRNA-seq protocol (Tecan) as recommended by the manufacturer. Libraries were quantified with NuQuant, quality checked using a TapeStation instrument (Agilent Technologies) and subjected to 52-bp paired-end sequencing on a NovaSeq 6000.
|
| |
|
| Library strategy |
ssRNA-seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
FASTQ-file generation and demultiplexing using bcl2fastq v. 2.20.0
STAR v2.7.2b was used to align sequence reads onto the GRCm38 mouse genome assembly, with the GENCODE version M25 gene model
reads were summarized onto genes by featureCounts v1.6.2 with the same gene model as when mapping, counting only fragments where both ends of the fragment were aligned
Assembly: mm10
Supplementary files format and content: featureCounts file containing gene-level mapped reads for the given sample
|
| |
|
| Submission date |
Oct 18, 2023 |
| Last update date |
May 14, 2024 |
| Contact name |
Lars Roed Ingerslev |
| E-mail(s) |
ingerslev@sund.ku.dk
|
| Organization name |
Copenhagen University
|
| Department |
NNF Center for Basic Metabolic Research
|
| Lab |
Integrative Physiology
|
| Street address |
Blegdamsvej 3B
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE245727 |
GLP-1 directed NMDA receptor antagonism for obesity treatment [II] |
| GSE245728 |
GLP-1 directed NMDA receptor antagonism for obesity treatment |
|
| Relations |
| BioSample |
SAMN37877023 |
| SRA |
SRX22130707 |
